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Updated: Jun 15, 2026

Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
[Polypronuclear zygotes and clinical pregnancy after IVF]
Hui-Rong Yin1, Xiao-Qiao Qian, Ling-Bo Cai
1Center of Human Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Objective:
To study the relationship between the percentage of polypronuclear zygotes and clinical pregnancy following IVF.
Methods:
We collected the data of 954 IVF cycles, and according the percentage of polypronuclear zygotes in the IVF cycles, allocated them to Groups A (without polypronuclear zygotes) , B (with < 30% polypronuclear zygotes) and C (with > or = 30% polypronuclear zygotes). Then we analyzed the relationship between the percentage of polypronuclear zygotes and the rate of clinical pregnancy.
Results:
Compared with Group A, Group C showed a significantly lower rate of clinical pregnancy (43.2% vs 28. 1%, P < 0.05), while Group B exhibited a markedly higher rate (43.2% vs 52.36%, P < 0.05) and obviously decreased polypronuclear zygote formation with the increase of age (35.6% vs 24.1%, P < 0.05).
Conclusion:
The percentage of polypronuclear zygotes in IVF cycles may serve as a prognostic indicator of the clinical outcome.
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