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Updated: Jun 15, 2026
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Determination of sperm DNA integrity by improved sperm chromatin structure assay test]
Jing Cai1, Pei-Yan Liang, Biao Yin
1Institute of Reproduction and Genetics, Shenzhen Zhongshan Urological Hospital, Shenzhen, Guangdong 518000, China. caijing0228@sina.com
Objective:
To search for a simple, quick and accurate method for the sperm DNA integrity test by modifying the conventional sperm chromatin dispersion (SCD) test, and to investigate the reference value range of normal sperm DNA integrity by the improved SCD assay.
Methods:
We modified the conventional SCD test in its procedures, reagents preparing and utensils to be used, compared the improved method with the conventional one, and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test.
Results:
An improved SCD test was established, and no statistical differences were found between the results of the modified and conventional methods. The reference value range was determined by the improved SCD assay for normal sperm DNA integrity, which was < 32.58% for abnormal sperm DNA integrity.
Conclusion:
The improved SCD test could detect sperm DNA integrity more quickly, and it reduced the cost in reagents.
