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Related Concept Videos

Erythropoiesis01:14

Erythropoiesis

Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
Erythropoiesis01:14

Erythropoiesis

Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

Developmental niches for embryonic erythroid cells.

Joan Isern1, Stuart T Fraser, Zhiyong He

  • 1Department of Medicine (Division of Hematology and Medical Oncology), Mount Sinai School of Medicine, New York, NY 10029, USA.

Blood Cells, Molecules & Diseases
|February 26, 2010
PubMed
Summary
This summary is machine-generated.

Researchers tracked primitive erythroid cells (EryP) during mammalian embryogenesis using a novel fluorescent reporter mouse. This method allowed detailed monitoring of EryP development across multiple embryonic tissues.

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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
08:31

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Published on: November 4, 2022

Area of Science:

  • Developmental Biology
  • Hematopoiesis
  • Genetics

Background:

  • Primitive erythroid cells (EryP) are the initial differentiated cell type in mammalian embryogenesis.
  • EryP originate in the yolk sac (YS) and mature within the embryonic circulation.
  • Tracking EryP maturation is challenging due to their decreasing numbers relative to definitive erythroid cells (EryD) later in development.

Purpose of the Study:

  • To develop a method for tracking and quantifying primitive erythroid cells (EryP) during mammalian embryogenesis.
  • To monitor EryP development and differentiation across distinct embryonic niches.

Main Methods:

  • Utilized a transgenic fluorescent reporter mouse line.
  • Employed human epsilon-globin gene regulatory elements to drive lineage-specific histone-H2B::EGFP expression.
  • Labeled EryP chromatin for tracking and quantification of nuclei post-expulsion.

Main Results:

  • Successfully monitored primitive erythropoiesis in the yolk sac, embryonic circulation, and fetal liver.
  • Enabled tracking of EryP development from progenitor stage to terminal differentiation.
  • Quantified EryP nuclei following their expulsion from maturing cells.

Conclusions:

  • The developed transgenic reporter system is effective for studying primitive erythropoiesis.
  • This method provides new insights into the maturational stages of EryP in vivo.
  • Facilitates detailed analysis of EryP development across key embryonic hematopoietic sites.