Related Experiment Video
Updated: Jun 15, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Microbiological and physical quality changes in vacuum loader cups associated with the use of various sanitizing
1USDA, Agricultural Research Service, Egg Safety and Quality Research Unit, Athens, GA 30605, USA. Deana.Jones@ars.usda.gov
Abstract:
Studies were conducted to determine the effects of various sanitizing compounds on the microbial and physical quality of shell egg processing vacuum loader cups. The sanitizing compounds used were as follows: sterile, distilled water; 200 microL/L of sodium hypochlorite; 200 microL/L of calcium hypochlorite; and 200 microL/L of peracetic acid. In the microbial inoculation study, cups were inoculated with Enterobacter cloacae because it was the most common isolate from a commercial study examining the flora found on vacuum loader cups. In all 3 replicates, aerobic plate counts and Enterobacteriaceae levels were similar for the clean control cups and the cups from the 2 chlorine treatments. Physical quality was measured via serial static compression testing using texture profile analysis. The serial compression mimicked the movement of the vacuum loader cups on the processing line. The strength of the vacuum loader cups was enhanced with exposure to any sanitizer treatment, including distilled water, compared with the controls throughout the 20 applications of the sanitizers. Durometer measurements were not consistent in monitoring vacuum loader cup quality and were determined to not be effective assessments for this application. The use of 200 microL/L of sodium hypochlorite or 200 microL/L of calcium hypochlorite successfully reduced microbial contaminants, had a positive effect on vacuum loader cup physical quality, and should be considered when developing sanitation programs for shell egg processing facilities.
Related Concept Videos
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Physical Methods for Controlling Microbial Growth: Temperature
Methods for Controlling Microbial Growth

