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A simple and efficient microassay method for titration of interferon.

H Ishitsuka, Y Nomura, K Takano

Microbiology and Immunology

|October 20, 1977

Summary

A new microassay method simplifies interferon titration using microtest plates. This efficient technique accurately measures interferon activity in small sample volumes, making it suitable for large-scale studies.

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Area of Science:

Virology
Immunology
Biochemistry

Background:

Interferon (IFN) is crucial for antiviral defense.
Accurate titration of IFN is essential for research and clinical applications.
Existing titration methods can be time-consuming or require large sample volumes.

Purpose of the Study:

To develop a simple, efficient, and scalable microassay for interferon titration.
To validate the microassay against established methods like plaque reduction.

Main Methods:

Utilized L929 cells and vesicular stomatitis virus (VSV) in microtest plates.
Interferon activity measured by 3H-uridine incorporation and reduction of extracellular radioactive RNA after VSV-induced cell lysis.
Quantified radioactivity in cold TCA-insoluble material in culture fluid.

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Main Results:

The microassay demonstrated high throughput for hundreds of samples in 2-3 days.
Interferon titers obtained were comparable to the plaque reduction method.
Assay sensitivity detected interferon activity >1 NIH reference unit/ml with 0.025 ml sample volume.

Conclusions:

The developed microassay is a simple, efficient, and cost-effective method for interferon titration.
This high-throughput assay is suitable for large-scale screening and research.
The method provides reliable interferon activity measurements with minimal sample requirements.