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Related Concept Videos

Burn Injuries01:22

Burn Injuries

Burn injuries occur when the skin and underlying tissues are damaged due to exposure to heat, electricity, chemicals, radiation, or friction. They can vary in severity, from minor superficial burns to severe deep burns that can be life-threatening.
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...

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Related Experiment Video

Updated: Jun 15, 2026

Rat Burn Model to Study Full-Thickness Cutaneous Thermal Burn and Infection
08:40

Rat Burn Model to Study Full-Thickness Cutaneous Thermal Burn and Infection

Published on: August 23, 2022

Circulating proteasomes after burn injury.

Matthias Majetschak1, Siegfried Zedler, Jacqueline Romero

  • 1Department of Surgery, Burn and Shock Trauma Institute, Loyola University Chicago Stritch School of Medicine, Maywood, Illinois, USA.

Journal of Burn Care & Research : Official Publication of the American Burn Association
|February 26, 2010
PubMed
Summary

Elevated 20S proteasome levels in burn patients indicate tissue damage and may aid in diagnosing inhalation injury. However, these levels do not reliably predict patient outcomes like organ failure or survival.

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Last Updated: Jun 15, 2026

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Area of Science:

  • Biochemistry
  • Trauma Surgery
  • Biomarker Discovery

Background:

  • Proteasomes are crucial cellular complexes involved in protein degradation.
  • Circulating proteasomes have been implicated in various disease states, but their role in burn injury is not well-defined.

Purpose of the Study:

  • To investigate elevated circulating proteasomes in burn patients.
  • To correlate proteasome levels with injury severity, organ failure, and clinical outcomes.

Main Methods:

  • Plasma samples from burn patients and healthy controls were analyzed for 20S and 26S proteasome levels using ELISA.
  • Proteasome activity was measured using a specific peptide substrate and inhibitor.
  • Clinical data including %TBSA burned, inhalation injury, sepsis, organ failure, and survival were collected.

Main Results:

  • Plasma 20S proteasome concentrations were significantly higher in burn patients on admission compared to controls, peaking on day 0 and returning to baseline by day 30.
  • Elevated 20S proteasomes correlated with inhalation injury and %TBSA burned in patients without inhalation injury.
  • 20S proteasome levels effectively discriminated the presence of inhalation injury but did not predict sepsis, organ failure, or survival.

Conclusions:

  • Circulating 20S proteasome serves as a biomarker for tissue damage in burn patients.
  • While not predictive of clinical outcomes, 20S proteasome plasma concentrations may assist in diagnosing inhalation injury.