Related Experiment Video
Updated: Jun 15, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
Published on: January 19, 2019
Hydroxyl-functionalized DNA: an efficient orthogonal protecting strategy and duplex stability
1School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, China.
Abstract:
Tert-butyldiphenylsilyl (TBDPS) was found to be an effective orthogonal protecting strategy for the 5-substituted hydroxyl groups of de novo synthesized deoxyuridine analogues 1-3 and 7-(3-hydroxypropynyl)- of 8-aza-7-deazadeoxyadenosine 4 for their incorporation into oligodeoxynucleotides by phosphoramidite chemistry. It could be completely cleaved under normal and ultra-mild deprotection conditions applied to DNA synthesis, without extra cleaving operation. The new phosphoramidites were coupled as usual with high yields. The new modified oligodeoxynucleotides were characterized by MALDI-TOF and enzymatic cleavage analysis. The thermal stability and conformation of these hydroxyl-functionalized DNA duplexes were evaluated.
More Related Videos
Related Concept Videos
Overview of DNA Repair
Chemically...
Single-Strand DNA Binding Proteins
Homologous Recombination
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Fixing Double-strand Breaks

