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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: Jun 15, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

HILIC methods in pharmaceutical analysis.

Bieke Dejaegher1, Yvan Vander Heyden

  • 1Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel, Brussels, Belgium.

Journal of Separation Science
|February 26, 2010
PubMed
Summary
This summary is machine-generated.

This review covers hydrophilic interaction liquid chromatography (HILIC) applications in pharmaceutical analysis, focusing on various stationary phases (SPs) like silica and polymer-based columns. It compares HILIC methods and discusses advantages, drawbacks, and validation for drug analysis.

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Last Updated: Jun 15, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
11:17

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
05:46

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Hydrophilic interaction liquid chromatography (HILIC) is a powerful separation technique.
  • HILIC utilizes a polar stationary phase (SP) and a mobile phase with a high organic solvent content.
  • It is particularly useful for analyzing polar compounds that are poorly retained in reversed-phase liquid chromatography.

Purpose of the Study:

  • To review and discuss hydrophilic interaction liquid chromatography (HILIC) applications in pharmaceutical analysis.
  • To categorize HILIC applications based on the type of stationary phase (SP) employed.
  • To compare HILIC methods with other chromatographic techniques and evaluate their advantages and disadvantages.

Main Methods:

  • The review categorizes HILIC applications based on stationary phase types: silica-based (bare-silica, chemically bonded, monolithic) and polymer-based (particle, monolithic).
  • Applications are discussed in relation to the specific SP used.
  • Comparisons are made with alternative HILIC methods and other separation modes where applicable.

Main Results:

  • Silica-based columns, particularly bare-silica, are the most frequently used SPs in pharmaceutical HILIC applications.
  • Polymer-based columns, though less common, also offer viable HILIC separation options.
  • The review systematically analyzes the performance and suitability of different SPs for various pharmaceutical analyses.

Conclusions:

  • HILIC is a versatile technique for pharmaceutical analysis, with stationary phase selection being critical for method performance.
  • Understanding the advantages and limitations of different HILIC stationary phases allows for optimized method development and validation.
  • Further research and application of HILIC, especially with novel stationary phases, will continue to advance pharmaceutical analysis.