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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 15, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Published on: January 7, 2019

Large-scale Ficoll gradient separations using a commercially available, effectively closed, system.

William E Janssen¹, Albert Ribickas, Laura V Meyer

¹Department of Blood and Marrow Transplant, Moffi tt Cancer Center, Tampa, Florida 33612, USA. William.Janssen@Moffitt.org

|February 27, 2010

Summary

This summary is machine-generated.

A new method using the Haemonetics Cell Saver5 instrument offers an efficient and closed system for clinical-scale Ficoll separation. This approach improves cell recovery and purity for cell therapy manufacturing, overcoming limitations of traditional methods.

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Area of Science:

Cellular biology
Biotechnology
Medical device engineering

Background:

Traditional Ficoll density separation is cumbersome and poses contamination risks for clinical-scale cell therapy manufacturing.
Existing automated systems like Sepax are limited in availability and costly.
Efficient and scalable cell separation is critical for producing cell-based therapeutics.

Purpose of the Study:

To develop and evaluate a novel, closed-system Ficoll separation protocol for clinical-scale cell preparation.
To assess the efficacy of the Haemonetics Cell Saver5 instrument for Ficoll density gradient separation.
To determine the feasibility of using a repurposed surgical blood salvage device for cell therapy manufacturing.

Main Methods:

A Ficoll separation protocol was developed utilizing the Haemonetics Cell Saver5, a surgical blood salvage and wash instrument.
The system employs standard blood bags and tubing with single-use components, creating a closed system.
Thirty-seven separation processes were analyzed, measuring depletion of red blood cells (RBC) and polymorphonuclear leukocytes (PMN), and recovery of mononuclear cells (MNC) and CD14+ monocytes.

Main Results:

The protocol achieved significant depletion of RBC (88.4%) and PMN (86.9%).
Mononuclear cell (MNC) recovery was 63.6%, with CD14+ monocyte recovery at 75.3%.
Total cell recovery was 49.2% from an average starting count of 14.6 x 10^9 cells.

Conclusions:

The Haemonetics Cell Saver5 provides a cost-effective and efficient solution for clinical-scale Ficoll separations.
The closed-system approach minimizes contamination risk, suitable for cell therapy manufacturing.
This method offers a simple, FDA-approved (for blood salvage) instrument for investigational cell separation needs.