Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Polytene Chromosomes02:04

Polytene Chromosomes

11.4K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
11.4K
The Ratio of X Chromosome to Autosomes02:45

The Ratio of X Chromosome to Autosomes

10.1K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.  
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
10.1K
Meiosis II02:02

Meiosis II

51.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
51.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Chaos to Care: Personalized AI for Early Cardiac Arrhythmia Warning.

medRxiv : the preprint server for health sciences·2026
Same author

Auditable cross-instrument detection of unusual multivariate psychiatric response configurations using a semantically aligned covariance subspace.

medRxiv : the preprint server for health sciences·2026
Same author

When Genes Fan the Flames: α5 Nicotinic Receptor Variants Amplify Cigarette Smoke-Driven Inflammation in COPD.

American journal of respiratory cell and molecular biology·2026
Same author

Foundation Model for Biological Temporal Data Dynamics with Experimental Validation.

Research square·2026
Same author

Single-stage "squeeze play" strategy for the clipping of seven aneurysms: illustrative case.

Journal of neurosurgery. Case lessons·2026
Same author

A mdg4 retrotransposon screen for X-linked female sterile alleles and its relationship with the transcription factor OVO.

G3 (Bethesda, Md.)·2026

Related Experiment Video

Updated: Apr 15, 2026

Preparation of Drosophila S2 cells for Light Microscopy
09:39

Preparation of Drosophila S2 cells for Light Microscopy

Published on: June 4, 2010

23.2K

Expression in aneuploid Drosophila S2 cells.

Yu Zhang1, John H Malone, Sara K Powell

  • 1Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.

Plos Biology
|February 27, 2010
PubMed
Summary

Gene copy number and expression in aneuploid cells were analyzed in Drosophila. Dosage compensation mechanisms were identified, revealing how cells regulate gene expression despite altered DNA copy numbers.

More Related Videos

Use of Drosophila S2 Cells for Live Imaging of Cell Division
06:17

Use of Drosophila S2 Cells for Live Imaging of Cell Division

Published on: August 23, 2019

9.1K
Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
07:10

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Published on: December 20, 2024

1.3K

Related Experiment Videos

Last Updated: Apr 15, 2026

Preparation of Drosophila S2 cells for Light Microscopy
09:39

Preparation of Drosophila S2 cells for Light Microscopy

Published on: June 4, 2010

23.2K
Use of Drosophila S2 Cells for Live Imaging of Cell Division
06:17

Use of Drosophila S2 Cells for Live Imaging of Cell Division

Published on: August 23, 2019

9.1K
Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
07:10

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Published on: December 20, 2024

1.3K

Area of Science:

  • Genetics
  • Molecular Biology
  • Cell Biology

Background:

  • Aneuploidy, an abnormal chromosome number, can disrupt cellular function due to imbalanced gene dosage.
  • The relationship between gene copy number variations and gene expression levels in aneuploid cells remains poorly understood.

Purpose of the Study:

  • To investigate genome-wide gene copy number and transcript abundance in aneuploid Drosophila S2 cells.
  • To elucidate the mechanisms of gene expression dosage compensation in the context of aneuploidy.

Main Methods:

  • DNA sequencing (DNA-Seq) to determine gene copy number.
  • RNA sequencing (RNA-Seq) to quantify transcript abundance (gene expression).
  • Analysis of aneuploid Drosophila S2 cells with a specific male genotype (2X;4A).

Main Results:

  • Drosophila S2 cells exhibited aneuploidy across >43 Mb of the genome, with copy numbers ranging from one to five.
  • Both X chromosomes and autosomes displayed expression dosage compensation.
  • X chromosome expression showed fixed-fold compensation, with precise dosage achieved only at a specific X-to-autosome ratio; feed-forward regulation was dependent on the Male Specific Lethal complex.

Conclusions:

  • Expression dosage compensation plays a crucial role in mitigating the effects of altered DNA copy number in aneuploid cells.
  • The X chromosome employs both fixed and dose-dependent compensation strategies.
  • Feed-forward regulation is a key component of X chromosome dosage compensation in Drosophila.