Related Experiment Video
Updated: Jun 15, 2026
07:14
Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
Published on: May 16, 2025
Biomineralized composite substrates increase gene expression with nonviral delivery
Rameshwar R Rao1, Jiawei He, J Kent Leach
1Department of Biomedical Engineering, University of California, Davis, California 95616, USA.
Journal of Biomedical Materials Research. Part A
|February 27, 2010
Summary
Biomineralized coatings on biodegradable substrates significantly enhance nonviral gene delivery efficiency in human mesenchymal stem cells (MSCs). This approach improves DNA and polyethyleneimine (PEI) uptake, offering a promising strategy for gene therapy.
Area of Science:
- Biomaterials Science
- Gene Therapy
- Cell Biology
Background:
- Gene transfer efficiency is crucial for therapeutic applications.
- Extracellular matrix and microenvironment significantly influence cellular activities impacting gene transfer.
- Current strategies primarily focus on vector development for DNA delivery.
Purpose of the Study:
- To test if a biomineralized coating on biodegradable substrates affects transgene expression.
- To investigate the impact of substrate rigidity and mineral coverage on nonviral gene delivery.
Main Methods:
- Fabrication of thin films from polymeric microspheres, with biomineralized films soaked in modified simulated body fluid.
- Culture of human mesenchymal stem cells (MSCs) on mineralized and nonmineralized substrates.
- Transfection of MSCs using plasmid DNA condensed with linear polyethyleneimine (PEI).
Main Results:
- Biomineralized films were significantly more rigid and exhibited widespread mineral coverage.
- Increased transgene expression was observed in cells cultured on biomineralized films compared to nonmineralized films across all charge ratios.
- Enhanced uptake of both PEI and DNA by cells was noted on mineralized substrates.
Conclusions:
- Biomineralized coatings on biodegradable substrates can effectively enhance nonviral gene delivery efficiency.
- This approach modulates gene delivery by improving cellular uptake of genetic material and vectors.
- The findings suggest a novel strategy to improve the efficacy of nonviral gene delivery systems.
More Related Videos
Related Concept Videos
02:11Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
00:59Gene Therapy
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...