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Updated: Jun 15, 2026
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Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis
Published on: March 19, 2019
Melittin induces apoptotic features in Candida albicans
1School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea.
Biochemical and Biophysical Research Communications
|March 2, 2010
Summary
Melittin, an antimicrobial peptide, induces antifungal effects by triggering apoptosis in Candida albicans. This involves increased reactive oxygen species (ROS) and DNA fragmentation, revealing a novel mechanism of action.
Area of Science:
- Mycology
- Biochemistry
- Cell Biology
Background:
- Melittin is a known antimicrobial peptide.
- Its membrane-active properties are well-established.
- The specific mechanisms of its antifungal activity require further elucidation.
Purpose of the Study:
- To investigate the mechanism by which Melittin exerts its antifungal effect on Candida albicans.
- To determine if Melittin induces apoptosis in yeast cells.
- To identify key cellular events associated with Melittin-induced cell death.
Main Methods:
- Exposure of Candida albicans to Melittin.
- Measurement of reactive oxygen species (ROS) production using DHR-123 staining.
- Detection of apoptosis markers via FITC-annexin V, TUNEL, and DAPI fluorescence microscopy.
Main Results:
- Melittin treatment led to increased ROS production in Candida albicans.
- FITC-annexin V staining indicated phosphatidylserine externalization.
- TUNEL and DAPI staining confirmed DNA and nuclear fragmentation, indicative of apoptosis.
Conclusions:
- Melittin demonstrates antifungal activity against Candida albicans.
- This antifungal effect is mediated through the induction of apoptosis.
- Melittin's mechanism involves ROS generation and subsequent DNA/nuclear fragmentation.
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