Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency07:08

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

1.4K
The present protocol describes the reprogramming of Pancreatic Ductal Adenocarcinoma (PDAC) and normal pancreatic ductal epithelial cells into induced pluripotent stem cells (iPSCs). We provide an optimized and detailed, step-by-step procedure, from preparing lentivirus to establishing stable iPSC...
1.4K
Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research07:26

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research

2.4K
This protocol presents a physiologically relevant tumor-on-a-chip model to perform high-throughput basic and translational human cancer research, advancing drug screening, disease modeling, and personalized medicine approaches with a description of loading, maintenance, and evaluation procedures.
2.4K
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies06:27

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

9.5K
The goal of this protocol is to establish a 3D in vitro model to study the differentiation of cancer-associated fibroblasts (CAFs) in a tumor bulk-like environment, which can be addressed in different analysis systems, such as immunofluorescence, transcriptional analysis and life cell...
9.5K
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer08:20

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

6.0K
The purpose of the method presented here is to show how microenvironment microarrays (MEMA) can be fabricated and used to interrogate the impact of thousands of simple combinatorial microenvironments on the phenotype of cultured...
6.0K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

9.7K
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are...
9.7K
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

10.0K
Here we present a detailed protocol to detect both senescent and pluripotent stem cells in the skeletal muscle upon injury while inducing in vivo reprogramming. This method is suitable for evaluating the role of cellular senescence during tissue regeneration and reprogramming in vivo.
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes Following Ultrasound-guided Percutaneous Cryoneurolysis for the Treatment of Spasticity.

American journal of physical medicine & rehabilitation·2026
Same author

Deletion of 9p drives B-ALL through heterozygous inactivation of Pax5 and Cd72 in preleukemic cells.

JCI insight·2026
Same author

PD-1 Expression Promotes Immune Evasion in B-ALL.

Hematology reports·2025
Same author

<i>Sox9</i> prevents retinal degeneration and is required for limbal stem cell differentiation in the adult mouse eye.

eLife·2025
Same author

Multimodal imaging in the diagnosis of myocardial abscesses in disseminated aspergillosis.

European heart journal. Case reports·2025
Same author

A Multifaceted Digital Intervention for the Prevention of Type 2 Diabetes Mellitus in Primary Care (PREDIABETEXT): Cluster Randomized Trial.

Journal of medical Internet research·2025

Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.4K

Physiological cellular reprogramming and cancer.

Fernando Abollo-Jiménez1, Rafael Jiménez, Cesar Cobaleda

  • 1Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer, CSIC/Universidad de Salamanca, Campus M. Unamuno s/n, Salamanca, Spain.

Seminars in Cancer Biology
|March 2, 2010
PubMed
Summary

Cancer research often overlooks cell differentiation, focusing instead on uncontrolled proliferation. This review explores how cellular reprogramming and plasticity are key to understanding tumor initiation and cancer biology.

More Related Videos

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

2.4K
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Published on: October 26, 2017

10.0K

Related Experiment Videos

Last Updated: Jan 19, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.4K
Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

2.4K
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Published on: October 26, 2017

10.0K

Area of Science:

  • Oncology
  • Developmental Biology
  • Cellular Reprogramming

Background:

  • Traditional cancer research prioritizes uncontrolled cell proliferation.
  • This focus has led to underemphasizing alterations in normal cell differentiation processes.
  • Oncogenic changes reprogram cellular fate, initiating pathological developmental programs.

Purpose of the Study:

  • To review the latest findings in cellular reprogramming.
  • To discuss the implications of cellular reprogramming for tumor biology.
  • To highlight the link between cancer, reprogramming, and cellular plasticity.

Main Methods:

  • Literature review of recent research in induced pluripotency.
  • Analysis of molecular mechanisms underlying tumor initiation and differentiation.
  • Synthesis of findings on cellular plasticity and oncogenic reprogramming.

Main Results:

  • Cellular reprogramming and plasticity are crucial for tumor initiation.
  • Specific oncogenes and cellular contexts enable tumoral reprogramming.
  • Induced pluripotency research offers new insights into cancer mechanisms.

Conclusions:

  • Altered differentiation and cellular reprogramming are central to cancer biology.
  • Understanding cellular plasticity is essential for novel cancer therapies.
  • Reprogramming offers a new perspective on cancer initiation and progression.