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Updated: Jan 30, 2026

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Published on: December 5, 2019
An in planta technique for cis-/trans-stereochemical analysis of jasmonoyl isoleucine
Shigeru Tamogami1, Ganesh Kumar Agrawal, Randeep Rakwal
1Department of Biological Production, Laboratory of Biologically Active Compounds, Akita Prefectural University, Akita 010-0195, Japan.
Abstract:
A novel technique for determining the cis-/trans-stereochemistry of jasmonoyl-isoleucine by coupling its alcoholic derivatives by sodium borohydride with high performance liquid chromatography-tandem mass spectrometry is described. Resolving cis- and trans-stereochemistry of the jasmonates in Achyranthes plants exposed to airborne (exogenous) trans-d(2)MeJA was demonstrated as an example. This novel application firmly establishes for the first time that trans-d(2)MeJA is converted exclusively into trans-JA-Ile in Achyranthes leaves, whereas the subsequent de novo biosynthesized JA-Ile possesses cis-stereochemistry. The method is simple, reproducible and could be employed for in vivo cis-/trans-stereochemistry analysis of jasmonates in plants.
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