Phenotypic analysis of human bone marrow macrophages
1Department of Haematology, Monash Medical Centre, Prince Henry's Hospital, Melbourne, Victoria, Australia.
Bone marrow macrophages have been isolated for phenotypic analysis by their ability to bind erythroblasts in erythroid clusters. In situ, when labeled for CD68 antigen, they are seen to form an arborizing network uniformly distributed throughout the hemopoietic marrow. Marrow macrophages isolated within erythroid clusters are acid phosphatase and alpha-naphthol butyrate esterase positive. Immunophenotypically, they are highly reactive for CD4, phagocytic receptors FcRI, II, and III, for HLA-Dr and CD31, as well as for the integrins CD11a, CD11c, and CD18, but negative for CD35 and transferrin receptor epitopes. Comparison of their phenotype with blood monocytes and cultured macrophages reveals significant differences, which indicate that marrow macrophages are specialized, differentiated mononuclear phagocytes that selectively associate with developing erythroblasts.
Bone marrow macrophages have been isolated for phenotypic analysis by their ability to bind erythroblasts in erythroid clusters. In situ, when labeled for CD68 antigen, they are seen to form an arborizing network uniformly distributed throughout the hemopoietic marrow. Marrow macrophages isolated within erythroid clusters are acid phosphatase and alpha-naphthol butyrate esterase positive. Immunophenotypically, they are highly reactive for CD4, phagocytic receptors FcRI, II, and III, for HLA-Dr and CD31, as well as for the integrins CD11a, CD11c, and CD18, but negative for CD35 and transferrin receptor epitopes. Comparison of their phenotype with blood monocytes and cultured macrophages reveals significant differences, which indicate that marrow macrophages are specialized, differentiated mononuclear phagocytes that selectively associate with developing erythroblasts.
Related Concept Videos
01:40Bone Remodeling
01:55Bone Structure
01:22Bone Marrow Sampling and Transplants
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
01:35Pedigree Analysis
02:27Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
01:27Compact Bone
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...