Related Experiment Video
Updated: Jun 15, 2026

Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Osteogenesis by gradually expanding the interface between bone surface and periosteum enhanced by bone marrow stem
Koichiro Sato1, Naoto Haruyama, Yoshinaka Shimizu
1Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan. koichiro@mail.tains.tohoku.ac.jp
Objective:
We investigated whether administration of mesenchymal stem cells (MSCs) promotes bone formation at the gap created by periosteal distraction.
Study Design:
A mesh plate was placed subperiosteally in rabbit parietal bones. Following elevation of the mesh plate, rabbit MSCs were administered into the gap. Controls received phosphate-buffered saline (PBS). The volume, height, bone mineral density (BMD), and bone mineral content (BMC) of newly formed bone were examined using microcomputed tomography. Histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry for type I collagen and osteocalcin.
Results:
The experimental group showed significantly increased volume, height, BMD, and BMC in newly formed bone tissues at the gaps compared with the control group (P < .05). The newly formed bone tissues showed both type I collagen and osteocalcin expression in the MSC-administration group.
Conclusion:
Mesenchymal stem cell administration may be useful to induce osteogenesis at sites of periosteal distraction.
Related Concept Videos
Bone Remodeling
Bone Formation by Endochondral Ossification
Bone Formation by Intramembranous Ossification
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into...

