Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

Ion Exchange

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nickel chelating functionalization of graphene composite for metal affinity membrane isolation of lysozyme.

Journal of materials chemistry. B·2020

Same author

In situ growth of β-FeOOH nanorods on graphene oxide with ultra-high relaxivity for in vivo magnetic resonance imaging and cancer therapy.

Journal of materials chemistry. B·2020

Same author

A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin.

Journal of materials chemistry. B·2020

Same author

A novel organic-inorganic hybrid polyoxometalate for the selective adsorption/isolation of β-lactoglobulin.

Journal of materials chemistry. B·2020

Same author

The regulation of hydrophilicity and hydrophobicity of carbon dots via a one-pot approach.

Journal of materials chemistry. B·2020

Same author

Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin.

Journal of materials chemistry. B·2020

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Ionic liquid-polyvinyl chloride ionomer for highly selective isolation of basic proteins.

Yang Shu¹, Xu-Wei Chen, Jian-Hua Wang

¹Research Center for Analytical Sciences, Box 332, Chemistry Building, Northeastern University, Shenyang 110004, China.

|March 2, 2010

Summary

New hydrophilic ionic liquid-polyvinyl chloride (PVC) hybrids selectively adsorb basic proteins like hemoglobin, offering improved biocompatibility. This material shows promise for protein isolation and purification applications.

More Related Videos

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Published on: October 27, 2023

Related Experiment Videos

Last Updated: Jun 15, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Published on: October 27, 2023

Area of Science:

Materials Science
Biochemistry
Separation Science

Background:

Polyvinyl chloride (PVC) is a common polymer with limitations in specific biomolecule adsorption.
Ionic liquids offer unique properties but require effective immobilization for practical applications.
Developing selective protein adsorption materials is crucial for biochemical research and diagnostics.

Purpose of the Study:

To synthesize and characterize novel hydrophilic ionic liquid-polyvinyl chloride (PVC) hybrids.
To evaluate the selective protein adsorption capabilities of these hybrids, particularly for basic proteins.
To assess the biocompatibility and practical utility of the hybrids in protein isolation.

Main Methods:

Immobilization of N-methylimidazole (N-mim) onto PVC chains in toluene to form NmimCl-PVC hybrids.
Characterization using FT-IR, 1H NMR, surface charge analysis, and elemental analysis.
Protein adsorption and elution experiments using various basic and acidic proteins in different buffer systems.

Main Results:

The NmimCl-PVC hybrids exhibited varying immobilization ratios (4.3%–15.1%) based on N-mim/PVC molar ratio.
Excellent selectivity for basic proteins (lysozyme, cytochrome c, hemoglobin) with high adsorption efficiencies (up to 98%).
Negligible adsorption of acidic proteins (BSA, transferring, IgG) and efficient recovery of adsorbed basic proteins (up to 89%).
Maintained hemoglobin activity post-adsorption and elution, demonstrating improved biocompatibility.
Successfully demonstrated selective isolation of hemoglobin from human whole blood.

Conclusions:

Hydrophilic ionic liquid-PVC hybrids demonstrate superior selective adsorption of basic proteins over acidic ones.
The immobilization ratio directly influences the selectivity and efficiency of protein adsorption.
These hybrids offer enhanced biocompatibility and are suitable for practical applications in protein purification and isolation, such as hemoglobin from blood.