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Related Experiment Video

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In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
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Human mast cells express intracellular TRAIL.

Beata Berent-Maoz1, Souzan Salemi, David Mankuta

  • 1Department of Pharmacology and Experimental Therapeutics, Institute for Drug Research, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

Cellular Immunology
|March 2, 2010
PubMed
Summary

Human mast cells (MC) produce intracellular Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL). Stimuli like LPS and IFN-gamma up-regulate TRAIL expression but do not trigger its release from these cells.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Human mast cells (MC) express functional Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) death receptors.
  • Understanding TRAIL expression and localization in MC is crucial for immune response modulation.

Purpose of the Study:

  • To assess mRNA and protein expression of TRAIL in cord blood-derived MC (CBMC) and HMC-1 cells.
  • To evaluate spontaneous and induced TRAIL release from MC.
  • To determine the intracellular localization of TRAIL in MC.

Main Methods:

  • Quantitative analysis of TRAIL mRNA and protein levels.
  • Stimulation of MC with lipopolysaccharide (LPS), interferon-gamma (IFN-gamma), and IgE-dependent activation.
  • Immunofluorescence microscopy to determine protein localization.

Main Results:

  • TRAIL protein was detected intracellularly in CBMC, but not extracellularly.
  • TRAIL was not found within MC granules.
  • IFN-gamma and LPS treatments increased intracellular TRAIL expression in CBMC but did not induce release.
  • HMC-1 cells showed a different TRAIL expression pattern compared to CBMC.

Conclusions:

  • Human mast cells produce and express intracellular TRAIL.
  • The intracellular localization of TRAIL in MC is stimulus-independent.
  • These findings provide insights into the intracellular handling of TRAIL within human mast cells.