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J Paul Robinson1

  • 1Purdue University, 1203 West State Street, Discovery Park, West Lafayette, IN 47907, USA. jpr@flowcyt.cyto.purdue.edu

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Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...