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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Updated: Jun 15, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

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Using ChIP-based technologies to identify epigenetic modifications in disease-relevant cells.

Jeffrey Falk1

  • 1Aviva Systems Biology, 11025 Roselle Street, Suite 100, San Diego, CA 92121, USA. jfalk@avivasysbio.com

Idrugs : the Investigational Drugs Journal
|March 2, 2010
PubMed
Summary
This summary is machine-generated.

Epigenetic modifications like DNA methylation influence gene expression and are linked to diseases. Chromatin immunoprecipitation (ChIP) technologies map these epigenetic changes genome-wide, aiding disease research.

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A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
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Last Updated: Jun 15, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Area of Science:

  • Epigenetics and Molecular Biology
  • Genomics and Bioinformatics

Background:

  • Epigenetic modifications, including DNA methylation and histone modifications, play a crucial role in regulating gene expression.
  • A growing body of evidence links aberrant epigenetic patterns to the development and progression of various human diseases, notably cancer.

Purpose of the Study:

  • To review the fundamental nature of epigenetic modifications.
  • To discuss the capabilities and applications of chromatin immunoprecipitation (ChIP)-based technologies for epigenome analysis.
  • To highlight the utility of these methods in identifying epigenetic regulatory mechanisms in medically relevant fields such as oncology and immunology.

Main Methods:

  • Utilizes chromatin immunoprecipitation (ChIP) coupled with high-throughput sequencing (ChIP-Seq) and arrays (ChIP-chip).
  • Genome-wide mapping of epigenetic marks like DNA methylation, histone methylation, and histone acetylation.
  • Comparative epigenetic mapping to correlate modifications with physiological changes in disease-relevant cells.

Main Results:

  • ChIP-based technologies enable high-resolution, genome-wide profiling of the epigenome.
  • These methods facilitate the identification of specific epigenetic modifications associated with gene expression changes in disease contexts.
  • Comparative analyses reveal correlations between epigenetic alterations and physiological states in cancer, stem cells, and T-cells.

Conclusions:

  • Epigenetic modifications are critical regulators of gene expression with significant implications for human health and disease.
  • ChIP-based technologies are powerful tools for dissecting the epigenome and understanding its role in disease.
  • Further application of these methods promises to uncover novel epigenetic targets for therapeutic intervention in cancer and immunological disorders.