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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Published on: November 13, 2021

Quantitation in mass-spectrometry-based proteomics.

Waltraud X Schulze1, Björn Usadel

  • 1Max Planck Institute for Molecular Plant Physiology, Golm, Germany. wschulze@mpimp-golm.de

Annual Review of Plant Biology
|March 3, 2010
PubMed
Summary
This summary is machine-generated.

Mass-spectrometry-based proteomics is a key plant biology tool. This review covers quantitative proteomics strategies, challenges, and data processing for deeper insights into plant protein function.

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Area of Science:

  • Plant Biology
  • Proteomics
  • Biochemistry

Background:

  • Mass-spectrometry-based proteomics has become routine in plant biology labs.
  • Early studies focused on protein identification, but there's a need for quantitative analysis.
  • Transitioning to quantitative proteomics presents both opportunities and challenges.

Purpose of the Study:

  • To review the current state of differential proteomics in plants.
  • To discuss strategies for quantitative protein analysis.
  • To highlight challenges and data processing approaches in plant proteomics.

Main Methods:

  • Overview of stable isotope-based and label-free quantitative proteomics strategies.
  • Discussion of statistical assessment methods for differential proteomics.
  • Exploration of data processing techniques for quantitative proteomic data.

Main Results:

  • Several differential proteomics strategies exist, each with strengths and limitations.
  • Incomplete proteome coverage and limited dynamic range are major challenges.
  • Effective data processing is crucial for precise quantitative analysis.

Conclusions:

  • Quantitative proteomics offers novel insights into plant protein dynamics and function.
  • Addressing current limitations is key to advancing plant proteomics.
  • This review provides a guide to current strategies and data processing in differential plant proteomics.