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Related Experiment Videos

A sensitive method for isolating Fusobacterium necrophorum from faeces.

G R Smith1, S A Barton, L M Wallace

  • 1Institute of Zoology, Zoological Society of London, Regent's Park.

Epidemiology and Infection
|April 1, 1991
PubMed
Summary
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A new method enhances Fusobacterium necrophorum infectivity using specific bacteria, enabling sensitive detection in faeces. This advance aids in identifying F. necrophorum (Fusobacterium necrophorum) in animals, with potential for epidemiological studies.

Area of Science:

  • Microbiology
  • Veterinary Science
  • Infectious Diseases

Background:

  • Fusobacterium necrophorum isolation is challenging due to contamination and high infective dose.
  • Efficient selective media for F. necrophorum are lacking.
  • Faecal and soil samples often contain F. necrophorum in low numbers.

Purpose of the Study:

  • To develop a sensitive method for detecting and isolating faecal strains of F. necrophorum type A.
  • To overcome limitations in current F. necrophorum isolation techniques.
  • To explore enhanced infectivity for improved detection.

Main Methods:

  • Subcutaneous injection of faeces (5% w/v in broth) with Actinomyces (Corynebacterium) pyogenes or Staphylococcus aureus into mice.
  • Mice were pretreated with clostridial antitoxins.

Related Experiment Videos

  • Identification of F. necrophorum microscopically at lesion edges and isolation on a selective medium.

    • Main Results:

    • Staphylococcus aureus showed slightly higher efficiency than A. pyogenes in enhancing F. necrophorum infectivity.
    • The method detected as few as 80 F. necrophorum organisms/g of faeces.
    • F. necrophorum was detected in 3 of 16 wallabies examined.

    Conclusions:

    • A sensitive method for F. necrophorum detection and isolation has been established.
    • Enhanced infectivity using specific bacterial co-cultures improves detection limits.
    • The method has potential for epidemiological investigations of F. necrophorum infections.