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Related Experiment Videos

Methods for enumerating Escherichia coli in subtropical waters.

W H Cheung1, D K Ha, K Y Yeung

  • 1Environmental Protection Department, Southorn Centre, Wanchai, Hong Kong.

Epidemiology and Infection
|April 1, 1991
PubMed
Summary

Modified membrane filtration methods improve Escherichia coli (E. coli) detection in Hong Kong waters. The mLS-UA and mLS-GUD methods offer precise enumeration, with mLS-GUD enabling automated counting for routine environmental water analysis.

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Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Bacteriology

Background:

  • Standard membrane filtration methods for enumerating Escherichia coli (E. coli) require improvement in specificity for subtropical water conditions.
  • Hong Kong's subtropical waters present unique challenges for accurate bacterial indicator monitoring.

Purpose of the Study:

  • To modify the standard UK membrane filtration method for enhanced specificity in enumerating E. coli in Hong Kong's subtropical waters.
  • To evaluate the efficacy of two modified methods, mLS-UA and mLS-GUD, compared to traditional techniques.

Main Methods:

  • Incorporation of an in situ urease test (mLS-UA) or an in situ beta-glucuronidase test (mLS-GUD) into the membrane lauryl sulphate (mLS) method.
  • Comparison of the modified membrane filtration methods (mLS-UA) against the multiple tube technique for E. coli enumeration in beach waters.

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  • Assessment of false-positive error rates for both mLS-UA and mLS-GUD methods.

    • Main Results:

    • The mLS-UA and mLS-GUD methods demonstrated low false-positive error rates, ranging from 3-5%.
    • The membrane filtration (mLS-UA) method provided significantly more precise E. coli counts compared to the multiple tube technique in subtropical beach waters.
    • Automated counting of E. coli colonies is feasible with the mLS-GUD method.

    Conclusions:

    • The modified membrane filtration methods (mLS-UA and mLS-GUD) are highly specific for enumerating E. coli in subtropical waters.
    • The mLS-UA method is recommended as the method of choice for precise E. coli enumeration in subtropical beach waters.
    • The mLS-GUD method serves as a viable alternative for routine E. coli enumeration in environmental waters, offering the advantage of automated colony counting.