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Related Experiment Videos

A microassay method for neurotoxic esterase determinations.

L Correll1, M Ehrich

  • 1Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg 24061.

Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology
|January 1, 1991
PubMed
Summary

A new microassay effectively measures neurotoxic esterase (NTE) and acetylcholinesterase (AChE) activities in hen tissues. This method is validated and reduces assay time by 50% for organophosphorus ester research.

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Area of Science:

  • Toxicology
  • Biochemistry
  • Neuroscience

Background:

  • Organophosphorus esters can cause delayed neuropathy.
  • Neurotoxic esterase (NTE) inhibition is an early indicator of this toxicity.
  • Diisopropylphosphorofluoridate (DFP) is a model organophosphorus compound used in research.

Purpose of the Study:

  • To develop and validate a microassay for measuring neurotoxic esterase (NTE) and acetylcholinesterase (AChE) activities.
  • To assess DFP-induced esterase inhibition in hen spinal cord and brain tissues.
  • To evaluate the efficiency and advantages of the microassay method.

Main Methods:

  • A microtiter plate reader was used to measure NTE and AChE activities in hen tissues.
  • Diisopropylphosphorofluoridate (DFP) was administered to hens to induce esterase inhibition.

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  • The microassay was validated by comparing results with conventional assays.

    • Main Results:

    • DFP administration led to similar inhibition of both NTE and AChE activities.
    • DFP-induced NTE inhibition was not localized to specific spinal cord regions with notable pathology.
    • The microassay reduced assay time by 50% and required significantly less sample and reagent volume.

    Conclusions:

    • The developed microassay is a validated, efficient, and sensitive method for measuring NTE and AChE activities.
    • The microassay is suitable for small regional measurements and high-throughput analysis.
    • This method aids in the study of organophosphorus ester-induced neurotoxicity.