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[Complete sequence of the M segment of Amur virus in rodent from China]
Wei-dong Guo1, Wen-yi Zhang, Jia-fu Jiang
1Inner Mongolia Autonomous Region Center for Disease Control and Prevention, Huhehaote 010031, China.
Objective:
To study the complete sequence of M segment of Amur virus in rodents and to explore their molecular characteristics.
Methods:
Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software.
Results:
The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76 - 118.
Conclusion:
The complete sequence on M segment of Amur virus in rodent was first time identified in this country.
