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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular
Walter Arancio1, Maria C Onorati, Giosalba Burgio
1Dipartimento di Scienze Biochimiche, Istituto Telethon Dulbecco, Universitá degli Studi di Palermo, Palermo, Italy.
Genetics
|March 3, 2010
Summary
Researchers identified new factors that regulate ISWI ( இன்டர்னல் சிக்னலிங் விங்-இன்-ஸ்பாட் ) , an ATP-dependent chromatin remodeler crucial for DNA replication and transcription. This discovery reveals a conserved network of interacting cellular and nuclear factors.
Area of Science:
- Molecular Biology
- Genetics
- Chromatin Biology
Background:
- ISWI ( இன்டர்னல் சிக்னலிங் விங்-இன்-ஸ்பாட் ) is a key ATP-dependent chromatin remodeler involved in DNA replication, RNA transcription, and chromosome organization.
- The diverse functions of ISWI imply intricate regulatory mechanisms controlling its activity.
Purpose of the Study:
- To identify novel factors that antagonize (suppress) ISWI activity.
- To uncover the regulatory network surrounding ISWI function.
Main Methods:
- Development of a novel in vivo eye-based assay for screening genetic suppressors of ISWI.
- Genetic screening in Drosophila melanogaster to identify interacting factors.
Main Results:
- The screen identified an evolutionarily conserved network of cellular and nuclear factors that interact with ISWI.
- These factors had previously evaded detection through genetic and biochemical analyses.
Conclusions:
- ISWI activity is regulated by a complex and conserved network of interacting factors.
- The novel eye-based assay is effective for identifying ISWI-interacting proteins and understanding chromatin remodeling regulation.
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