Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 15, 2026

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
08:41

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy

Published on: June 13, 2025

A stretching device for high-resolution live-cell imaging.

Lawrence Huang1, Pattie S Mathieu, Brian P Helmke

  • 1Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.

Annals of Biomedical Engineering
|March 3, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sustainability on the Menu: Assessing the Role of Hospital Cafeteria Composting in Advancing Planetary Health Initiatives.

International journal of environmental research and public health·2026
Same author

Treatment Failure and Post-Artesunate Delayed Haemolysis in a Returned Traveller From Uganda With Partially Drug-Resistant Severe Plasmodium falciparum Malaria.

The Medical journal of Australia·2026
Same author

Greater ambient air pollution exposure is associated with worse respiratory symptoms in men and women with HIV and chronic lung disease: a cohort study.

Respiratory research·2025
Same author

Pulmonary Artery Endothelial Cells from Patients with Pulmonary Arterial Hypertension Exhibit Heterogeneous Responses to Their Mechanical Microenvironment.

American journal of respiratory cell and molecular biology·2025
Same author

Leveraging Datathons to Teach AI in Undergraduate Medical Education: Case Study.

JMIR medical education·2025
Same author

Bacteremia and Community-Acquired Pneumonia Caused by Pantoea stewartii Subspecies indologenes, Australia.

Emerging infectious diseases·2025
Same journal

A Physiologic Left Ventricle Flow Phantom for 4D Flow MRI Applications and CFD Verification.

Annals of biomedical engineering·2026
Same journal

Pulsatile Hemodynamics of Prehypertension and Hypertension: Associations with Pressure and Sex.

Annals of biomedical engineering·2026
Same journal

A Pressure Difference-Based Strategy for Blood Oxygen Control in Membrane Oxygenators: Reduced Modeling, Computational Simulation, and Exploratory In Vivo Evaluation.

Annals of biomedical engineering·2026
Same journal

Multidirectional Optical Bone Densitometry Using a Simulation-Based Machine Learning Model: Experimental Validation with Bone Phantoms.

Annals of biomedical engineering·2026
Same journal

Numerical Study of Human Torso Mechanical Response and Injury Assessment Under Blast Loading with Bulletproof Protection.

Annals of biomedical engineering·2026
Same journal

Immediate and Mid-Long-Term Effects of Foot Orthoses on Gait Biomechanics and Clinical Characteristics in Medial Knee Osteoarthritis: A Systematic Review and Meta-analysis.

Annals of biomedical engineering·2026
See all related articles

A new microscopy device enables live-cell imaging of cellular responses to mechanical strain. It reveals how cells transmit and focus mechanical stress, offering insights into dynamic structural remodeling.

Area of Science:

  • Cell Biology
  • Biophysics
  • Bioengineering

Background:

  • Investigating cellular responses to substrate strain is crucial for understanding mechanobiology.
  • Existing devices often limit high-resolution live-cell imaging during mechanical stretching.
  • Real-time analysis of structural dynamics in living cells under strain remains a challenge.

Purpose of the Study:

  • To introduce a novel stretch device optimized for high-resolution live-cell imaging.
  • To enable detailed analysis of cellular structural remodeling under controlled mechanical strain.
  • To investigate the transmission and focusing of mechanical stress within cellular structures.

Main Methods:

  • Developed a novel stretch device compatible with inverted microscopes for live-cell imaging.

More Related Videos

Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
09:20

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Related Experiment Videos

Last Updated: Jun 15, 2026

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
08:41

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy

Published on: June 13, 2025

Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
09:20

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

  • Applied constant magnitude or cyclic stretch at physiological levels to cells on elastic membranes.
  • Utilized interchangeable indenters for equibiaxial and uniaxial stretch profiles.
  • Tracked fluorescent microspheres to analyze strain profiles and quantified cellular responses using microscopy.

    • Main Results:

    • The device reproducibly applied defined stretch profiles (equibiaxial and uniaxial).
    • Mechanical strain efficiently transmitted through the extracellular matrix and focal adhesions to endothelial cells.
    • Reduced strain transmission to the intermediate filament network (vimentin) was observed, with strain focusing at discrete sites.
    • Actin-mediated edge ruffling showed no desensitization to repeated mechanostimulation.

    Conclusions:

    • The novel stretch device is a valuable tool for live-cell microscopy of mechanical strain effects.
    • It facilitates the measurement of dynamic structural remodeling in response to mechanical stimuli.
    • The findings provide new insights into how cells perceive and respond to mechanical forces at the structural level.