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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: Jun 15, 2026

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
06:26

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Published on: January 6, 2023

Probing protein colloidal behavior in membrane-based separation processes using spectrofluorometric Rayleigh

Rand Elshereef1, Hector Budman, Christine Moresoli

  • 1Dept. of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada N2L 3G1.

Biotechnology Progress
|March 3, 2010
PubMed
Summary
This summary is machine-generated.

Rayleigh light scattering can predict membrane fouling in protein separation. Increased protein aggregation, indicated by higher scattering, correlates with reduced membrane flux and increased fouling.

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Area of Science:

  • Biophysical Chemistry
  • Membrane Science
  • Separation Technology

Background:

  • Membrane fouling is a significant challenge in protein separation processes.
  • Understanding protein colloidal behavior is crucial for mitigating fouling.
  • Current methods for assessing colloidal behavior can be complex.

Purpose of the Study:

  • To explore the feasibility of using Rayleigh light scattering to correlate with membrane fouling.
  • To investigate the relationship between protein solution properties and membrane flux decline.
  • To assess Rayleigh scattering as a tool for probing protein colloidal behavior.

Main Methods:

  • Utilized dead-end ultrafiltration (UF) to measure membrane flux.
  • Employed multiwavelength Rayleigh scattering to assess protein solution colloidal behavior.
  • Analyzed protein solutions including beta-lactoglobulin under varying conditions.

Main Results:

  • Rayleigh scattering data showed good agreement with known colloidal behavior of beta-lactoglobulin.
  • Reduced Rayleigh scattering correlated with stable protein solutions and minimal fouling.
  • Increased Rayleigh scattering correlated with protein aggregation and significant flux decline.

Conclusions:

  • Rayleigh light scattering is a viable method for predicting membrane fouling in protein separations.
  • Protein aggregation, detected by scattering, is a key driver of membrane fouling.
  • This technique offers a promising approach to monitor and control fouling during filtration.