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Updated: Jun 15, 2026
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
Published on: May 5, 2014
[Study on gene delivery via the bile duct to rat liver using naked DNA particles]
Jin-hua Hu1, Biao Xu, Jing Chen
1Liver Failure Treatment and Research Center, 302 hospital of the PLA, Beijing 100039, China. hjh@redmail.com.cn
Objective:
To evaluate the efficacy of gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct using naked DNA.
Methods:
We evaluated regional gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct, using naked DNA, including pGL3, pCMV beta and Cy3 labeled CMV beta.
Results:
Gene expression was observed in right lateral lobe of both the damaged and the normal rats liver. The gene delivery efficiency was similar in two groups (P > 0.05). Gene expression was found in the right lateral lobe of damaged and normal livers. Fluorescence was observed in the region of the portal triads, and occasionally, in the lobule.
Conclusion:
Retrograde infusion of naked DNA via the bile duct is an effective way to deliver genes to in both damaged and normal rat liver.
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