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A rat monoclonal antibody against the chromatin remodeling factor CHD5

Saori Yoshimura1, Tomohiko Yoshimi, Yasuyuki Ohkawa

  • 1Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.

Hybridoma (2005)
|March 5, 2010
PubMed

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Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...