Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Key Molecular Events in PM<sub>2.5</sub>-Induced Lung Injury: Autophagy and Ferroptosis Mediated by the <i>miR-212-5p</i>/<i>RASSF1</i> Axis.

Cells·2026
Same author

Role of Second Halogen Atoms of Dihalobenzene in Controlling the Photoluminescence Properties of Single-Walled Carbon Nanotubes by Reductive Arylation.

ACS nanoscience Au·2026
Same author

Bulk Amorphous Alumina: The Density-Driven Interplay of Pentahedral Pyramids and Octahedra for High Dielectric Permittivity.

Journal of the American Chemical Society·2026
Same author

Topology-guided rotational dynamics of magnetic soliton assemblies under pulsed current.

Nature communications·2026
Same author

Current-Driven Nonreciprocal Response of Nonequilibrium Skyrmions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Differential Toxicity of Water-Soluble Versus Water-Insoluble Components of Cowshed PM2.5 on Ovarian Granulosa Cells and the Regulatory Role of Txnip in Overall Toxicity.

Antioxidants (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 15, 2026

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Local crystal structure analysis with several picometer precision using scanning transmission electron microscopy.

Koji Kimoto1, Toru Asaka, Xiuzhen Yu

  • 1National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. kimoto.koji@nims.go.jp

Ultramicroscopy
|March 5, 2010
PubMed
Summary
This summary is machine-generated.

Scanning transmission electron microscopy (STEM) enables picometer-scale crystal structure analysis. Advanced annular dark-field (ADF) imaging techniques improve resolution and accuracy for atomic measurements.

More Related Videos

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

Related Experiment Videos

Last Updated: Jun 15, 2026

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

Area of Science:

  • Materials Science
  • Physics
  • Chemistry

Background:

  • Accurate crystal structure analysis is crucial for understanding material properties.
  • Traditional electron microscopy techniques have limitations in achieving picometer precision.

Purpose of the Study:

  • To demonstrate high-precision local crystal structure analysis using scanning transmission electron microscopy (STEM).
  • To showcase advanced annular dark-field (ADF) imaging techniques for enhanced resolution and accuracy.

Main Methods:

  • Utilized software-based techniques for signal-to-noise ratio improvement and image distortion reduction.
  • Employed maximum-entropy deconvolution for enhanced resolution.
  • Quantified experimental parameters like specimen thickness and defocus.

Main Results:

  • Achieved local crystal structure analysis with picometer-level precision.
  • Demonstrated the effectiveness of advanced ADF imaging in improving image quality.
  • Identified the dependence of atom position measurement accuracy on the incoherent imaging approximation.

Conclusions:

  • Advanced ADF imaging in STEM allows for highly precise atomic-scale structural analysis.
  • Thin specimens and specific imaging conditions are necessary for deep-sub-angstrom accuracy.
  • This methodology offers new possibilities for nanoscale materials characterization.