Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cardiomyopathy in vitro.

A Wada, Y Inui, H Fushimi

    Recent Advances in Studies on Cardiac Structure and Metabolism
    |May 26, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Cardiomyopathic hamster heart cells exhibit impaired sarcomere maturation and altered protein kinase activity compared to controls. These findings suggest underlying molecular differences affecting myofibril organization in heart disease.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Spironolactone inhibits the transcardiac extraction of aldosterone in patients with congestive heart failure.

    Journal of the American College of Cardiology·2000
    Same author

    Mycobacterium kansaii flexor tenosynovitis presenting as carpal tunnel syndrome.

    Journal of hand surgery (Edinburgh, Scotland)·2000
    Same author

    Adrenomedullin inhibits spontaneous and bradykinin-induced but not oxytocin- or prostaglandin F(2alpha)-induced periodic contraction of rat uterus.

    British journal of pharmacology·2000
    Same author

    Insufficient secretion of atrial natriuretic peptide at acute phase of myocardial infarction.

    Journal of applied physiology (Bethesda, Md. : 1985)·2000
    Same author

    Studies on ATPase(GTPase) intrinsic to E. coli ribosomes.

    Journal of biochemistry·2000
    Same author

    Inhibition by neuroprotective drug NS-7 of nicotine-induced 22Na(+) influx, 45Ca(2+) influx and catecholamine secretion in adrenal chromaffin cells.

    Brain research·2000

    Area of Science:

    • Cardiology
    • Cell Biology
    • Biochemistry

    Background:

    • Cardiomyopathy in hamsters presents as a progressive heart muscle disease.
    • Understanding the cellular and molecular basis of this condition is crucial for developing effective treatments.

    Purpose of the Study:

    • To investigate the morphological and biochemical differences between heart cells from cardiomyopathic and control hamsters.
    • To identify potential molecular mechanisms underlying the observed cardiac dysfunction.

    Main Methods:

    • Phase contrast, differential interference contrast, and electron microscopy of cultured heart cells.
    • Measurement of [14C] leucine incorporation into acid-insoluble fractions.
    • Analysis of erythrocyte membrane proteins using polyacrylamide gel electrophoresis.

    Related Experiment Videos

  • Assay of protein kinase activity and [3H] cAMP binding in erythrocyte ghosts.

    • Main Results:

    • Cultured cardiomyopathic heart cells showed decreased beating frequency and rhythm regularity.
    • Electron microscopy revealed impaired sarcomere maturation in diseased cells.
    • No significant difference in leucine incorporation was observed.
    • Differences in erythrocyte membrane protein composition and cAMP-sensitive protein kinase activity were detected between groups.

    Conclusions:

    • Cardiomyopathy in hamsters is associated with impaired sarcomere maturation and altered erythrocyte membrane protein kinase activity.
    • These findings suggest potential differences in subunit interactions and myofibril organization in diseased hearts.
    • The role of neurosecretory granule-like corpuscles remains undetermined but may be significant.