Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA methylation mediates the multiple sclerosis onset risk associated with HHV-6 DNA positivity.

Environmental epigenetics·2026
Same author

Somatic variation in multiple sclerosis: from blood to brain.

Current opinion in genetics & development·2026
Same author

Updated Health Economics Simulation Model of the Lifetime Progression of Multiple Sclerosis in an Australian Setting.

PharmacoEconomics - open·2026
Same author

Do sleep interventions improve sleep in MS? A systematic review and meta-analysis of activity monitor assessed outcomes.

Multiple sclerosis and related disorders·2026
Same author

Neurofilament Light Chain Concentration in the Prediction of Treatment Response in Multiple Sclerosis.

European journal of neurology·2026
Same author

Filial piety and fertility decisions, Thailand.

Bulletin of the World Health Organization·2026
Same journal

Genetically proxied inhibition of cholesterol-lowering drug targets and survival in HPV-positive and non-HPV driven head and neck cancer: a multicentre MR study.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Retraction: Menstrual Factors, Reproductive History and Liver Cancer Risk: Findings from a Prospective Cohort Study in Chinese Women.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Optimizing the Uptake and Completion of the Cervical Cancer Screening Continuum: Opportunity Knocks, Including at Home.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Sex Differences in Cancer Stage at Diagnosis of Nonreproductive Solid Organ Tumors in the United States, 2015 to 2022.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Evaluation of the 4Kscore in EPIC: Reply.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Evaluation of the 4Kscore in EPIC: Letter.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Saliva-derived DNA performs well in large-scale, high-density single-nucleotide polymorphism microarray studies.

Melanie Bahlo1, Jim Stankovich, Patrick Danoy

  • 1The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Victoria, Australia. bahlo@wehi.edu.au

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
|March 5, 2010
PubMed
Summary
This summary is machine-generated.

Saliva DNA is a viable alternative to blood DNA for large-scale genotyping studies like genome-wide association studies (GWAS). This research confirms high concordance in genotyping quality and quantity, supporting saliva DNA

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Related Experiment Videos

Last Updated: Jun 15, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Genome-wide association studies (GWAS) are crucial for identifying genetic variants associated with diseases.
  • These studies require substantial amounts of high-quality DNA from numerous individuals.
  • The source of DNA (e.g., blood vs. saliva) can impact genotyping efficiency and data quality.

Purpose of the Study:

  • To evaluate the suitability of saliva-derived DNA for high-density genotyping in a large-scale multiple sclerosis (MS) genome-wide association study.
  • To compare the performance of saliva DNA against blood DNA using key genotyping metrics.

Main Methods:

  • Genotyping was performed on the Illumina Infinium Hap370CNV DUO microarray for a multiple sclerosis GWAS.
  • DNA from 10 individuals was genotyped in duplicate using both saliva and blood samples.
  • Performance was assessed by comparing genotyping call rates and Illumina's GCScore (a DNA quality measure) between blood and saliva DNA.

Main Results:

  • High concordance in genotyping quality and quantity was observed between paired saliva and blood DNA samples.
  • Saliva DNA showed minimal loss in quality and quantity compared to blood DNA in the large GWAS cohort.
  • Blood samples exhibited greater variation in genotyping performance across different centers of origin.

Conclusions:

  • Saliva-derived DNA is a reliable and effective source for DNA in high-density single-nucleotide polymorphism microarray studies, including large-scale GWAS.
  • The findings support the broader use of saliva DNA in genetic research, offering a less invasive alternative to blood collection.