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Related Concept Videos

Imaging Studies I: Kidney, Ureter, and Bladder Studies01:28

Imaging Studies I: Kidney, Ureter, and Bladder Studies

Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
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The kidneys are a pair of bean-shaped organs in the human body that play a critical role in maintaining overall health. They filter out waste products from the blood, regulate blood pressure, maintain electrolyte balance, and stimulate the production of red blood cells.
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Related Experiment Video

Updated: Jun 15, 2026

Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging
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Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Published on: March 28, 2020

Tomographic quantification of branching morphogenesis and renal development.

Kieran M Short1, Mark J Hodson, Ian M Smyth

  • 1Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, Victoria 3800, Australia.

Kidney International
|March 5, 2010
PubMed
Summary

This study introduces a novel 3D imaging method for analyzing kidney development, revealing new insights into ureteric branching defects in Tgfbeta2(+/-) embryos and improving the study of renal developmental diseases.

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Last Updated: Jun 15, 2026

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Medical Imaging

Background:

  • Branching morphogenesis is crucial for kidney development but challenging to image in advanced stages.
  • Current methods like confocal microscopy are limited by tissue thickness and explant culture artifacts.
  • Ex vivo organ cultures lack vascularization and proper matrix, distorting ureteric branching.

Purpose of the Study:

  • To develop and validate a 3D imaging and quantification method for embryonic kidney branching.
  • To overcome limitations of current imaging techniques for studying renal development.
  • To analyze genetic influences on ureteric branching using this new approach.

Main Methods:

  • Utilized optical projection tomography for 3D imaging of ex vivo embryonic kidneys.
  • Developed custom software to quantify ureteric branching in 3D.
  • Assessed branching parameters including length, tip number, iterations, angles, and inter-tip distances.
  • Analyzed Tgfbeta2(+/-) embryos from E12.5 to E15.5.

Main Results:

  • Successfully imaged and quantified 3D ureteric branching in embryonic kidneys.
  • Quantified decreased branching in Tgfbeta2(+/-) embryos, differing from prior organ culture studies.
  • Identified a primary defect in renal pelvic formation in Tgfbeta2(+/-) embryos.
  • Demonstrated the utility of the method in studying genetic influences on kidney development.

Conclusions:

  • The developed 3D imaging and analysis method overcomes limitations of traditional techniques.
  • This approach provides improved throughput and observation of in vivo branching states.
  • It offers a valuable tool for investigating the basis of renal developmental diseases and genetic mutations affecting kidney formation.