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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
In vitro selection of RNA aptamers to a small molecule target
Vlad Codrea1, Michelle Hayner, Bradley Hall
1Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
Current Protocols in Nucleic Acid Chemistry
|March 5, 2010
Abstract:
This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection.

