Related Experiment Video

Updated: Jun 15, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

[Matrix metalloproteinase 9 presence in adhesive intraperitoneal processes]

R A Pintilie1, Daniela Constantinescu, Carmen Grigoriu

  • 1Facultatea de Medicină, Disciplina de Obstetrică-Ginecologie, Universitatea de Medicină si Farmacie "Gr.T. Popa" Iaşi.

Revista Medico-Chirurgicala a Societatii De Medici Si Naturalisti Din Iasi
|March 6, 2010
PubMed
Abstract

Insights

Matrix metalloproteinases (MMP) are involved in tissue remodeling. This study found increased MMP9 levels in chronic peritoneal adhesions, suggesting their role in the progression and potential resolution of these adhesions.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pathology

Background:

  • Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteolytic enzymes crucial for various physiological and pathological processes.
  • MMPs play significant roles in tissue remodeling, wound healing, and angiogenesis.

Purpose of the Study:

  • To investigate the presence of MMP9 in chronic peritoneal adhesions.
  • To correlate MMP9 levels with the connective tissue remodeling process in intraperitoneal adhesions.

Main Methods:

  • Tissue samples from 6 women with peritoneal adhesions and normal peritoneum were analyzed.
  • Immunohistochemistry and image analysis were used to detect and quantify MMP9 expression.

Main Results:

  • Peritoneal adhesions were primarily composed of fibrous tissue with collagenous components.
  • MMP9 levels were significantly higher in adhesions (26.5% positive area) compared to normal peritoneum (15.93% positive area).

Conclusions:

  • Chronic intraperitoneal adhesions, particularly those over 1 year old, exhibit advanced remodeling processes.
  • Elevated MMP9 expression in adhesions suggests its involvement in the progression and potential resolution of these fibrotic tissues.

More Related Videos

Detection of Functional Matrix Metalloproteinases by Zymography09:30

Detection of Functional Matrix Metalloproteinases by Zymography

Published on: November 8, 2010

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...