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Updated: Jun 15, 2026
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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
Polyacrylamide gel-based microarray: a novel method applied to the association Study between the polymorphisms of
Lu Cheng1, Qinyu Ge, Beili Sun
1State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
Journal of Biomedical Nanotechnology
|March 6, 2010
Summary
This study investigated the association between Brain-Derived Neurotrophic Factor (BDNF) gene variations and autism in the Chinese population. Findings suggest a potential link between specific BDNF polymorphisms and autism susceptibility.
Area of Science:
- Genetics
- Neuroscience
- Population Studies
Background:
- Single nucleotide polymorphisms (SNPs) are crucial for identifying genes associated with complex diseases.
- High-throughput SNP genotyping methods are essential for large-scale association studies.
Purpose of the Study:
- To investigate the association between Brain-Derived Neurotrophic Factor (BDNF) gene polymorphisms and autism in the Chinese population.
- To evaluate the efficacy of a polyacrylamide gel-based microarray for SNP genotyping in association studies.
Main Methods:
- Applied a polyacrylamide gel-based microarray with universal dual-color hybridization for trios-based association study.
- Genotyped four SNPs in patients with autism and their parents.
- Utilized single SNP and haplotype transmission disequilibrium tests (TDT).
Main Results:
- The C270T SNP in BDNF showed preferential transmission of the T allele in autistic individuals (TDT p < 0.001).
- Haplotype analysis also revealed a significant association involving the C270T polymorphism (TDT p < 0.05).
- The microarray method proved rapid, simple, and high-throughput for SNP genotyping.
Conclusions:
- Results suggest a potential association between BDNF gene polymorphisms and autism in the Chinese population.
- The developed microarray technology is effective and cost-efficient for large-scale genetic association studies of complex disorders.
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