Control and optimization of apheresis procedures in a COBE 2997 cell separator

Area of Science:

• Biomedical Engineering
• Medical Device Technology
• Cell Separation Science

Background:

• Clinical cell separators require precise control for efficient operation.
• Existing COBE Model 2997 systems lack complete computer control for automated adjustments.
• Optimizing separation parameters is crucial for maximizing therapeutic yields.

Purpose of the Study:

• To enhance the operational efficiency of the COBE Model 2997 clinical cell separator.
• To implement computer control for maintaining consistent product cell density.
• To investigate optimal operating regions for plateletpheresis and leukapheresis.

Main Methods:

• Modified the cell separator for complete computer control.
• Utilized an optical sensor for real-time product cell density detection.
• Employed a proportional-integral (PI) algorithm to adjust flow rates via microcomputer interface.
• Conducted a second-order factorial experimental design to assess operational parameters.

Main Results:

• Achieved stable product cell density maintenance without operator intervention in both Single Stage II and Dual Stage chambers.
• Identified optimal operating regions for plateletpheresis at moderate set points and high centrifuge RPM, yielding 63-70% efficiency.
• Determined optimal conditions for leukapheresis at high set points and RPM (>1200), achieving near 100% efficiency.

Conclusions:

• Computer-controlled cell separation significantly improves operational efficiency and product consistency.
• The modified system enables precise control for optimizing apheresis procedures.
• Specific operating parameters (set point, RPM) are critical for maximizing efficiency in platelet and white blood cell collection.