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Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

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Related Experiment Video

Updated: Jun 15, 2026

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

Electrostatic surface modifications to improve gene delivery.

Ron B Shmueli¹, Daniel G Anderson, Jordan J Green

¹Johns Hopkins University School of Medicine, Department of Biomedical Engineering, 400 N. Broadway, Smith Building 5017, Baltimore, MD 21231, USA.

Expert Opinion on Drug Delivery

|March 6, 2010

Summary

This summary is machine-generated.

Electrostatic surface modifications enhance biomaterials for improved gene delivery, stabilizing therapeutics and enabling targeted delivery of DNA and siRNA for advanced gene therapies.

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Last Updated: Jun 15, 2026

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

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Published on: September 12, 2011

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Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Published on: August 7, 2014

Area of Science:

Biomaterials Science
Gene Therapy
Nanotechnology

Background:

Gene therapy offers potential treatments for genetic diseases and cancer.
Effective gene delivery is crucial for therapeutic success.

Purpose of the Study:

To review biomaterials for gene delivery.
To focus on electrostatic surface modifications for enhanced gene delivery.
To highlight the role of electrostatic principles in multilayer fabrication.

Main Methods:

Review of biomaterials used in gene delivery.
Focus on electrostatic surface modifications (coatings, multilayers).
Discussion of layer-by-layer fabrication methods.

Main Results:

Electrostatic modifications stabilize therapeutics in vivo.
Targeting ligands and controlled release are enabled by these modifications.
Improved spatial control and delivery efficiency for nucleic acids (DNA, siRNA) in vitro and in vivo.

Conclusions:

Electrostatic coatings and multilayers represent a versatile approach for gene therapy enhancement.
This strategy promises to advance the development of more effective gene therapies.