Related Experiment Video
Updated: Jun 15, 2026

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
Level of chemical and microbiological contaminations in chili bo (paste)
Nurul Aqilah Mohd Zaini1, Hanis Hazeera Harith, Akanbi Taiwo Olusesan
1Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Abstract:
The objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). Fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for pH, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. Results showed that both moisture content and pH vary among samples. The concentrations of benzoic acid detected in chili bo were found to be in the range of 537 to 5,435 mg/kg. Nine of fifteen brands were found to exceed the maximum level permitted by the Malaysian Food Law in accordance with the Codex Alimentarius (1,000 mg/kg for benzoic acid). An apparent correlation between benzoic acid concentration and microbiological loads present in the chili bo was observed. The microbiological loads were found to be relatively low in the end products containing high amounts of benzoic acid. The heat-resistant (70 to 80 degrees C) microorganisms present in chili bo were identified as Ochrobacterum tritici, Stenotrophomonas rhizophila, Microbacterium maritypicum, Roseomonas spp., CDC group II-E subgroup A, Flavimonas oryzihabitans, and Pseudomonas aeruginosa, with M. maritypicum being the most frequently found (in 9 of 15 samples) microorganism. Most of these identified microorganisms were not known to cause foodborne illnesses.
Insights
Many chili paste brands exceed safe benzoic acid levels, potentially impacting microbial safety. Higher benzoic acid concentrations correlated with lower microbial loads in tested chili paste products.
Area of Science:
- Food Science
- Microbiology
- Analytical Chemistry
Background:
- Preservatives are crucial for extending the shelf life of food products like chili paste.
- Understanding microbial contamination and preservative levels is vital for food safety regulations.
Purpose of the Study:
- To assess benzoic acid levels and microbial contamination in commercial chili paste.
- To determine compliance with Malaysian Food Law and Codex Alimentarius standards.
Main Methods:
- Analysis of pH, moisture, and benzoic acid content in 15 chili paste brands.
- Quantification of aerobic, anaerobic, aerobic spore, and fungal loads.
- Identification of heat-resistant microorganisms.
Main Results:
- Benzoic acid levels ranged from 537 to 5,435 mg/kg, with 9 out of 15 brands exceeding the 1,000 mg/kg limit.
- A correlation was observed between high benzoic acid levels and reduced microbial loads.
- Heat-resistant bacteria like Microbacterium maritypicum were identified, but generally not associated with foodborne illness.
Conclusions:
- Several commercial chili paste brands violate benzoic acid regulations.
- Benzoic acid appears effective in controlling microbial growth in chili paste.
- Further monitoring is recommended to ensure food safety standards are met.
More Related Videos
07:07Controlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes
Published on: April 7, 2017
05:49Tracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example
Published on: March 5, 2014
Related Concept Videos
Sources of Food Contamination
Pasteurization and Food Preservation
Microbial Spoilage of Food
Methods of Controlling Food Spoilage
Microbes in the Production of Fermented Foods
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...