Related Experiment Video
Updated: Jun 15, 2026
07:48
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Published on: June 7, 2014
Optical properties of particle migration imaging film
Applied Optics
|March 6, 2010
Abstract:
The optical properties of an electrophotographic film comprised of closely packed submicron selenium spheres embedded in a polymer matrix were investigated. In one case the spheres were arranged in a monolayer; in a second the particles were dispersed throughout the polymer matrix. The optical transmission of these structures was measured as a function of wavelength and particle size and compared with physical models based on Mie theory.
Related Concept Videos
01:18Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...