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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 15, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Pulsed spontaneous Raman scattering technique for luminous environments.

A J Mulac, W L Flower, R A Hill

    Applied Optics
    |March 6, 2010
    PubMed
    Summary

    This study presents a new system combining gated photon counting, a cavity-dumped laser, and a multipass cell to significantly improve spontaneous Raman scattering measurements in bright light. The enhanced signal-to-background ratio enables detailed flame analysis.

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    Last Updated: Jun 15, 2026

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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    Published on: July 25, 2022

    Observation and Analysis of Blinking Surface-enhanced Raman Scattering
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    Observation and Analysis of Blinking Surface-enhanced Raman Scattering

    Published on: January 11, 2018

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    Area of Science:

    • Spectroscopy
    • Laser Physics
    • Combustion Diagnostics

    Background:

    • Spontaneous Raman scattering is crucial for analyzing gas composition and temperature.
    • Luminous background conditions severely limit signal-to-background ratio (SBR) in conventional Raman spectroscopy.
    • Existing methods struggle to provide accurate measurements in high-background environments like flames.

    Purpose of the Study:

    • To develop and demonstrate a novel system for enhancing spontaneous Raman scattering.
    • To overcome limitations posed by luminous backgrounds in spectroscopic analysis.
    • To enable precise temperature and density measurements in challenging environments.

    Main Methods:

    • Integration of a gated photon counting system with a cavity-dumped argon-ion laser.
    • Utilization of a multipass retroreflecting light cell to increase interaction length.
    • Application of a passband fitting technique for spectral data analysis.

    Main Results:

    • Achieved a 450-fold improvement in SBR compared to a single-pass continuous-wave system.
    • Gating and cavity dumping contributed a 30-fold SBR enhancement.
    • The retroreflecting light cell provided an additional 15-fold SBR improvement.
    • Successfully obtained temperature and density data from N(2) Q-branch spectra of a methane-air flame.

    Conclusions:

    • The developed system significantly enhances spontaneous Raman scattering in luminous backgrounds.
    • The combination of techniques offers a powerful tool for combustion diagnostics and other challenging spectroscopic applications.
    • The passband fitting technique provides a robust method for analyzing complex spectral data.