Initial process of diffusion of small molecules from blood vessels to the meninges in the young rat

Pathways taken by peripherally administered small molecules when entering the brain were investigated in 6-day-old rats by radioautography and fluorescence microscopy after intravenous administration and rapid freezing. L-[U-14C]Phenylalanine, [U-14C]sucrose and sodium fluorescein reached the brain within less than 5 seconds. These blood-borne molecules were found in the subarachnoid cisterns and the superficial parenchyma. These results suggest a special permeability of the arachnoid layer and/or the pial vessels. Phenylalanine alone reached the deep parenchyma because of the existence of a specific endothelial carrier.