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Exploring neoglycoprotein assembly through native chemical ligation using neoglycopeptide thioesters prepared via

Jonathan P Richardson1, Chung-Hei Chan, Javier Blanc

  • 1Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.

Organic & Biomolecular Chemistry
|March 6, 2010
PubMed

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Oligosaccharide Assembly01:24

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...