Related Experiment Video
Updated: Jun 15, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Hydroxyurea as an inhibitor of hepatitis C virus RNA replication
Akito Nozaki1, Manabu Morimoto, Masaaki Kondo
1Gastroenterological Center, Yokohama City University Medical Center, 4-57, Urafune-cho, Minami-ku, Yokohama 232-0024, Japan. akino@yokohama-cu.ac.jp
Abstract:
Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.
More Related Videos
Related Concept Videos
Hepatitis
Antiviral Nucleoside Inhibitors
Inhibitors of Viral Protein Synthesis
Viruses with RNA Genomes
Inhibitors Of Virion Release
Inhibitors of Virion Maturation and Assembly

