Related Experiment Video
Updated: Jun 15, 2026

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Searching for monooxygenases and hydrolases in bacteria from an extreme environment
Georgiana F da Cruz1, Célio F F Angolini, Luciana G de Oliveira
1Chemistry Institute, University of Campinas, UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil.
Abstract:
Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
More Related Videos
08:31Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
07:56A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms
Published on: January 12, 2024
Related Concept Videos
Deep Sea Microbial Ecology
Diversity of Archaea I
Overview of Archaea
Diversity of Archaea III
Hyperthermophilic Bacteria
Carbon-dioxide Fixation