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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

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Published on: September 22, 2011

Advances in clone selection using high-throughput bioreactors.

Bhargavi Kondragunta1, Jessica L Drew, Kurt A Brorson

  • 1Center for Advanced Sensor Technology, University of Maryland, Baltimore County, Baltimore, MD 21250, USA.

Biotechnology Progress
|March 6, 2010
PubMed
Summary
This summary is machine-generated.

Selecting mammalian cell clones using miniaturized stirred bioreactors improves production platform development. This high-throughput approach avoids missing high-producing clones, unlike traditional well plate methods.

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Mammalian cell culture is vital for producing biologics.
  • Current clone selection relies on well plates, which may not reflect optimal production conditions.
  • Scaling up clone selection for bioreactor evaluation is costly and limits throughput.

Purpose of the Study:

  • To introduce and evaluate miniaturized stirred high-throughput bioreactors (HTBRs) for mammalian cell clone selection.
  • To compare the efficacy of HTBRs against traditional well plate screening methods.
  • To demonstrate an improved clone selection strategy for bioproduction.

Main Methods:

  • Application of miniaturized stirred HTBRs (35 mL working volume) with noninvasive optical sensors.
  • Simultaneous testing of up to 12 subclones per HTBR run in a stirred environment.
  • Comparison of HTBR screening results with traditional stationary well plate culture data.

Main Results:

  • HTBRs enable simultaneous screening of multiple subclones under stirred conditions.
  • Clone selection based solely on well plate data risks overlooking superior producers.
  • Stirred bioreactor environments provide a more accurate representation of production conditions.

Conclusions:

  • Utilizing HTBRs offers a more reliable method for mammalian cell clone selection.
  • This high-throughput, stirred-culture approach enhances the development of robust cell culture production platforms.
  • The study advocates for bioreactor-based screening to optimize biopharmaceutical manufacturing.