Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin.

bioRxiv : the preprint server for biology·2026
Same author

Harnessing Higher-Dimensional Fluctuations in an Information Engine.

Physical review letters·2026
Same author

A Tetracycline-Inducible Promoter Replacement System for <i>Schizosaccharomyces pombe</i>.

microPublication biology·2025
Same author

In through the out door: A loop-binding-first model for topological cohesin loading.

BioEssays : news and reviews in molecular, cellular and developmental biology·2024
Same author

pomBseen: An automated pipeline for analysis of fission yeast images.

PloS one·2023
Same author

Information Engine in a Nonequilibrium Bath.

Physical review letters·2023
Same journal

Combining ultrastructure expansion microscopy with immunofluorescence and Oligopaint DNA FISH.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
Same journal

Bridging methodological gaps in avian cytogenetics: comprehensive and optimized protocols for chromosomal preparation in birds.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
Same journal

The role of satellite DNA-enriched heterochromatic variants in reproductive disorders: Insights from standardized cytogenetic analysis.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
Same journal

Cytogenomics of Myloplus tiete reveals conserved satellite DNAs since the Late Eocene in Serrasalmidae (Teleostei, Characiformes).

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
Same journal

Stable resynthesized Brassica napus lines show similar meiotic behaviour to established B. napus.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
Same journal

Characterization of neocentromeric marker chromosome derived from chromosome 11: a rare entity in four patients with acute leukemia.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Reconciling stochastic origin firing with defined replication timing.

Nicholas Rhind1, Scott Cheng-Hsin Yang, John Bechhoefer

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA. Nick.Rhind@umassmed.edu

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
|March 6, 2010
PubMed
Summary
This summary is machine-generated.

Stochastic origin firing in eukaryotic chromosomes can explain defined replication timing patterns. This occurs if origins have varying firing probabilities and if these probabilities increase throughout S phase.

More Related Videos

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Related Experiment Videos

Last Updated: Jun 15, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic chromosome replication occurs with precise timing.
  • The regulatory mechanisms governing replication timing remain largely unknown.
  • A discrepancy exists between population studies (defined average replication times) and single-molecule studies (stochastic origin firing).

Purpose of the Study:

  • To reconcile the apparent conflict between stochastic and defined origin firing patterns.
  • To propose a model explaining how stochasticity can lead to defined replication timing.
  • To suggest biochemically plausible mechanisms and experimental distinctions.

Main Methods:

  • A simple simulation model was developed.
  • The model incorporated varying origin firing probabilities.
  • The model analyzed the effect of increasing firing probability during S phase.

Main Results:

  • Stochastic origin firing can generate defined average replication timing patterns.
  • Two key criteria were identified: differential origin firing probabilities and probability increase during S phase.
  • Biochemically plausible mechanisms and experimental discriminators were proposed.

Conclusions:

  • Stochasticity in origin firing, coupled with specific regulatory conditions, can explain observed replication timing.
  • The proposed model provides a framework for understanding replication timing regulation.
  • Further experimental validation is suggested to distinguish between stochastic and deterministic models.