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Related Experiment Video

Updated: Jun 15, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

BglBricks: A flexible standard for biological part assembly.

J Christopher Anderson¹, John E Dueber, Mariana Leguia

¹Department of Bioengineering, University of California, Berkeley, CA 94720, USA. jcanderson@berkeley.edu

Journal of Biological Engineering

|March 9, 2010

Summary

A new DNA assembly standard, BglBricks, overcomes limitations of older methods like BioBricks. This advance enables seamless construction of protein fusions and other synthetic biology applications.

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Published on: December 1, 2017

Area of Science:

Synthetic Biology
Molecular Biology
Bioengineering

Background:

Standard biological parts are foundational for synthetic biology, enabling applications in bioenergy, therapeutics, and environmental remediation.
The original BioBricks assembly standard has limitations, particularly its unsuitability for protein fusions due to an unfavorable scar sequence encoding a stop codon.

Purpose of the Study:

To introduce BglBricks, a novel DNA composition standard designed to address the scar translation issue in protein fusion construction.
To provide a more flexible and robust platform for generating standard biological parts and automating DNA assembly.

Main Methods:

Development of the BglBricks standard utilizing BglII and BamHI restriction enzymes.
Demonstration of the BglBricks system's utility in constructing gene expression devices, protein fusions, and targeted DNA integrations.

Main Results:

The BglBricks system creates a 6-nucleotide scar sequence encoding glycine-serine, a suitable peptide linker for protein fusions.
Successful construction of constitutively active gene expression devices with diverse expression profiles.
Demonstrated ability to create chimeric, multi-domain protein fusions and integrate DNA sequences into specific genomic loci.

Conclusions:

The BglBricks standard offers a more flexible platform for biological part generation and DNA assembly automation.
Ongoing development of BglBrick assembly reactions and associated automation/bioinformatics tools aims to streamline genetic engineering.
This advancement facilitates the transformation of genetic engineering into a design-based discipline.