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Updated: Dec 28, 2025

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II
Yanan Ren1, Chunxi Zhang, Jingquan Zhao
1Laboratory of Photochemistry, Beijing National Laboratory of Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Abstract:
Chloride is an essential cofactor for photosynthetic water oxidation. However, its location and functional roles in active photosystem II are still a matter of debate. We have investigated this issue by studying the effects of Cl- replacement by Br- in active PSII. In Br- substituted samples, Cl- is effectively replaced by Br- in the presence of 1.2 M NaBr under room light with protection of anaerobic atmosphere followed by dialysis. The following results have been obtained. i) The oxygen-evolving activities of the Br--PSII samples are significantly lower than that of the Cl--PSII samples; ii) The same S2 multiline EPR signals are observed in both Br- and Cl--PSII samples; iii) The amplitudes of the visible light induced S1TyrZ* and S2TyrZ* EPR signals are significantly decreased after Br- substitution; the S1TyrZ* EPR signal is up-shifted about 8G, whereas the S2TyrZ* signal is down-shifted about 12 G after Br- substitution. These results imply that the redox properties of TyrZ and spin interactions between TyrZ* and Mn-cluster could be significantly modified due to Br- substitution. It is suggested that Cl-/Br- probably coordinates to the Ca2+ ion of the Mn-cluster in active photosystem II.
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